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目的观察依达拉奉(ED)对脓毒血症大鼠血及肝超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和自由基的影响。方法SD大鼠30只分为假手术组、手术对照组、ED治疗组(下称治疗组),手术对照组和治疗组均予盲肠结扎穿刺法制作大鼠脓毒血症模型,术前15 min及术后3 h各皮下注射乳酸左氧氟沙星20 mg/kg,治疗组于术前15 min及术后3 h各皮下注射ED 5 mg/kg,三组术后18 h采血和取肝组织测SOD、CAT、羟自由基(OH),并对肝组织进行显微镜、电镜检查。结果手术对照组大鼠肝组织显微镜下可见肝细胞浊肿,水样变,电镜下可见肝细胞核轻度固缩,内质网扩张,治疗组大鼠肝组织病理改变不明显。手术对照组血及肝组织OH活力与假手术组比较明显升高,差异有统计学意义,手术对照组血及肝组织SOD、CAT活力与假手术组比较明显降低,差异有统计学意义;治疗组血及肝组织OH活力与手术对照组比较明显降低,差异有统计学意义,治疗组血SOD、CAT活力与手术对照组比较明显升高,差异有统计学意义。结论ED能降低脓毒血症大鼠血和肝组织OH活力,升高血和肝组织SOD、CAT活力,提高清除自由基和抗氧化能力,具有保护肝功能作用,值得临床推鉴使用。
Objective To observe the effect of edaravone (ED) on blood serum and liver superoxide dismutase (SOD), catalase (CAT) and free radicals in septic rats. Methods Thirty SD rats were divided into sham operation group, operation control group, ED treatment group (the treatment group), operation control group and treatment group were given cecal ligation and puncture method to make rat sepsis model, preoperative 15 min and 3 h postoperatively, subcutaneous injection of levofloxacin 20 mg / kg and 15 mg / kg ED 5 mg / kg respectively in the treatment group and 18 h after operation. The levels of SOD, , CAT, hydroxyl radical (OH), and the liver tissue microscopy, electron microscopy. Results The hepatocyte cytoplasm and water samples were observed under microscope in the liver of the operation control group. The hepatic cell nuclear was slightly condensed and the endoplasmic reticulum was dilated by electron microscopy. The pathological changes of the liver tissue of the treated group were not obvious. There was a significant difference between the control group and the sham-operated group in the activity of OH and the activity of SOD in the liver and the liver in the control group. The activities of SOD and CAT in the blood and liver of the operation group were significantly lower than those in the sham-operated group The activities of OH and vitality of the blood and liver tissue were significantly lower than those of the control group, the difference was statistically significant. The activities of SOD and CAT in the blood of the treatment group were significantly higher than those in the control group, the difference was statistically significant. Conclusions ED can reduce the activity of OH in blood and liver tissue of septic rats, increase the activity of SOD and CAT in blood and liver tissues, improve the ability of scavenging free radicals and anti-oxidation, protect liver function and is worthy of clinical use.