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目的建立毒鼠强中毒患者的胃内容物、血液和尿液中快速检测毒鼠强的气质联用方法。方法运用C18小柱对胃内容物与血液进行除杂、净化后,通过气质联用仪上的大体积进样与程序升温进样,并利用小口径毛细管色谱柱HP-5MS(0.18 mm×20 m,0.18μm)进行检测,用选择离子模式对m/z 92、132、212、240进行采集。结果从样品前处理到确证完成全过程耗时约20 min。结论该方法具操作简易快速、定性准确、灵敏度高等特点,能应用于应急检验,为患者抢救工作节省了宝贵的时间。
Objective To establish a method for the rapid detection of tetramine in the gastric contents, blood and urine in patients with tetramine poisoning. Methods C18 column was used to purify the gastric contents and blood. After purifying, the sample was injected through a large volume of gas chromatograph and programmed temperature, and the capillary column HP-5MS (0.18 mm × 20 m, 0.18 [mu] m), m / z 92, 132, 212, 240 were collected using selected ion mode. Results from the sample pretreatment to confirm the completion of the entire process takes about 20 min. Conclusion The method has the characteristics of simple and quick operation, high accuracy and high sensitivity, which can be used in emergency inspection and save precious time for the salvage work of patients.