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本研究探讨大鼠肝部分切除65％和32％后1、2、3、7、14天期间,胰岛细胞数目和分泌活动的变化。取胰尾,用醛品红,Grimelius 法和 Hellerstrom—Hellman 法分别显示胰岛 B、A 和 D 细胞,计算上述三种岛细胞染色深度和分泌颗柱数量百分数,作为判断岛细胞分泌活动的指标。结果如下:1、A、B、D 三种岛细胞的数目均无明显改变,2、65％肝邵分切除后,胰岛细胞分泌活动的变化是:术后第1、2天,B 细胞分泌减弱,A 细胞稍强;术后第3天,B 细胞的分泌活动明显增强,A 细胞分泌继续增强,D 细胞分泌一直保持正常水平;术后第7天,B 细胞的分泌大为减弱,到术后第14天尚未恢复正常,在此期间,A 细胞分泌仍加强,D 细胞分泌也增强,3、32％肝部分切除后,岛细胞分泌活动的变化情况基本如上,只是变化的幅度较小和进程较快,其中以 B 细胞最明显。上述变化均与肝再生过程密切相伴。B细胞的变化似乎直接与肝再生相关,A 和 D 细胞可能起协同作用。
This study was to investigate the changes of islet cell number and secretory activity in rats at 1, 2, 3, 7, and 14 days after partial hepatectomy at 65% and 32%. The pancreatic islets B, A and D cells were displayed by the method of algin magenta, Grimelius method and Hellerstrom-Hellman method, respectively. The depths of the three islet cells and the percentage of the number of the secretory granules were calculated as indexes for judging the secretory activity of the islet cells. The results are as follows: 1, There was no significant change in the number of A, B and D island cells. The changes of pancreatic islet secretion after 2,65% hepatectomy were: the first and second postoperative days, B cells secrete Weakened, A cells slightly stronger; 3 days after surgery, B cell secretion increased significantly, A cell secretion continued to increase, D cell secretion has remained normal; on the 7th day after the B cell secretion was greatly reduced to The 14th day after operation did not return to normal. During this period, the secretion of A cells was still enhanced and the secretion of D cells was also enhanced. The changes of secretory activity of island cells were almost the same after 3,32% partial hepatectomy, but only slightly changed And the process faster, of which the most obvious B cells. The above changes are closely associated with the process of liver regeneration. B cell changes seem to be directly related to liver regeneration, A and D cells may play a synergistic role.