论文部分内容阅读
目的探讨孕中期羊水细胞染色体分析在产前诊断胎儿染色体异常中的价值。方法选取杭州地区2009年10月至2012年12月在杭州市第一人民医院产前诊断中心行羊水穿刺的2151例妊娠19-26周孕妇,行羊膜腔穿刺,取羊水细胞进行培养,常规收获,显带,并作核型分析。结果成功培养的2084例羊水染色体中,共检出异常核型77例,检出率为3.69%,检出多态172例,检出率为8.25%。不同年龄组孕妇间染色体异常检出率比较无统计学差异(P>0.05)。结论孕中期羊水细胞染色体核型分析安全,有效,可靠,是产前诊断的重要手段。
Objective To investigate the value of metaphase amniotic fluid cell chromosome analysis in the prenatal diagnosis of fetal chromosomal abnormalities. Methods A total of 2151 pregnancies, 19-26 weeks pregnant women who underwent amniocentesis in the prenatal diagnosis center of Hangzhou First People’s Hospital from October 2009 to December 2012 in Hangzhou were enrolled in this study. They were subjected to amniocentesis by amniocentesis, , Banding, and karyotype analysis. Results Of the 2084 cases of amniotic fluid cultured successfully, 77 cases of abnormal karyotype were detected, the detection rate was 3.69%. 172 cases were detected polymorphism, the detection rate was 8.25%. There was no significant difference in the detection rate of chromosomal abnormalities between pregnant women of different age groups (P> 0.05). Conclusion The chromosomal karyotypic analysis of amniotic fluid cells during the second trimester of pregnancy is safe, effective and reliable. It is an important method of prenatal diagnosis.