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实验选用4个细菌性条斑病分离菌和12个白叶枯病分离菌,用改良的Hayward培养基培养。用叶条斑病病原菌P_xt_2和白叶枯病原菌H_(384)制取抗血清。取经24小时琼脂斜面培养的细菌,制成蛋白质浓度为675微克/毫升的含盐细菌悬浮液。将细菌悬浮液与同体积的Freund完全佐剂混合并匀化。每周1次用它给白兔作皮下注射。于第四周末取兔血,用试管凝集法测抗血清滴定度。选取滴定度为2560的抗血清用于免疫电泳实验。在2.7×7.5厘米玻片上涂布3毫米厚的
Four bacterial leaf streak isolates and 12 bacterial leaf blight isolates were selected and cultured in a modified Hayward medium. Antisera were prepared from the leaf spot pathogen P_xt_2 and the bacterial leaf blight pathogen H_ (384). Bacteria cultured on agar slants for 24 hours were used to prepare salt-containing bacterial suspensions having a protein concentration of 675 μg / ml. The bacterial suspension is mixed with the same volume of Freund’s complete adjuvant and homogenized. Use it once a week for white subcutaneous injection. Rabbit blood was taken at the end of the fourth week, and the titer of antisera was measured by tube agglutination. Antiserum with a titer of 2560 was chosen for the immunoelectrophoresis experiment. Apply 3 mm thick on a 2.7 x 7.5 cm glass slide