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Aim:To investigate whether tanshinone ⅡA could improve the effect of UWsolution for skeletal muscle preservation and to determine the dose range oftanshinone ⅡA providing optimal protection during ischemia and reperfusion.Methods:Ischemic rat limbs were perfused with UW solution or UW plus tanshinoneⅡA (UW+T,0.05,0.1,or 0.2 mg/mL) for 0.5h before reperfusion;controls (I/R)received no perfusion.Serum creatine phosphokinase (CPK),aspartate aminotrans-ferase (AST),and lactate dehydrogenase (LDH) were measured pre-ischemia andafter reperfusion (2-h,4-h,and 6-h).Muscle water content,superoxide dismutase(SOD),malondialdehyde (MDA),adenosine triphosphatase (ATPase) were as-sessed pre-reperfusion and after 6-h reperfusion.Intercellular adhesion molecule-1(ICAM-1) and apoptosis were detected after 6-h reperfusion.Reperfusion bloodflow was monitored during reperfusion period.Results:UW and UW+T pre-vented luxury perfusion during reperfusion and inhibited ICAM-1 expression andapoptosis after 6-h reperfusion.Serum CPK,AST,and LDH levels in UW ratswere significantly less than those in controls after 2-h reperfusion (no difference,4-h or 6-h reperfusion).After 4-h ischemia,there were significant differences inwater content,MDA,SOD,and ATPase between UW and controls,but no differ-ence after 6-h reperfusion.All tests with UW+T rats were significantly differentfrom control results at corresponding durations.Higher tanshinone doses im-proved results.Conclusion:UW plus tanshinone ⅡA increased protection againstI/R injury,suggesting that tanshinone ⅡA has clinical value.
Aim: To investigate whether tanshinone ⅡA could improve the effect of UWsolution for skeletal muscle preservation and to determine the dose range oftanshinone ⅡA providing optimal protection during ischemia and reperfusion.Methods: Ischemic rat limbs were perfused with UW solution or UW plus tanshinoneⅡA (UW + T, 0.05,0.1, or 0.2 mg / mL) for 0.5h before reperfusion; controls (I / R) received no perfusion.Serum creatine phosphokinase (CPK), aspartate aminotrans-ferase (AST), and lactate dehydrogenase (LDH) were measured pre-ischemia andafter reperfusion (2-h, 4-h, and 6-h) .Muscle water content, superoxide dismutase (SOD), malondialdehyde (MDA), adenosine triphosphatase (ATPase) were as-sessed pre-reperfusion and after 6-h reperfusion.Intercellular adhesion molecule-1 (ICAM-1) and apoptosis were detected after 6-h reperfusion. Reperfusion blood flow was monitored during reperfusion period. Results: UW and UW + T pre-vented luxury perfusion during reperfusion and inhibited ICAM -1 expression andapoptosis afte r 6-h reperfusion. Serum CPK, AST, and LDH levels in UW rats were significantly less than those in controls after 2-h reperfusion (no difference, 4-h or 6-h reperfusion) significant differences inwater content, MDA, SOD, and ATPase between UW and controls, but no differ-ence after 6-h reperfusion.All tests with UW + T rats were significantly differentfrom control results at corresponding durations.Higher tanshinone doses im-proved results .Conclusion: UW plus tanshinone ⅡA increased protection againstI / R injury, suggesting that tanshinone ⅡA has clinical value.