在全球艾滋病发病率不断上升而现有治疗方法却难以普及的情况下,研究有效的HIV-1疫苗有重要意义。这种DNA疫苗采用病原体本身的基因为免疫原,具有与活病毒疫苗相同的免疫活性,又避免了后者固有的危险性,在体内可诱导产生MHC Ⅰ型及Ⅱ型反应。有效的HIV-1疫苗应能诱导人体产生强大的细胞免疫反应,因此作者进行了定量检测HIV-1 DNA疫苗诱导健康人产生T细胞反应的Ⅰ期临床试验。 In the context of the global AIDS epidemic rising but existing treatment methods are difficult to popularize, the study of effective HIV-1 vaccine is of great significance. The DNA vaccine adopts the gene of the pathogen itself as an immunogen, has the same immunological activity as the live virus vaccine, avoids the inherent danger of the latter, and induces MHC type I and type II reactions in vivo. A valid HIV-1 vaccine should induce a powerful cellular immune response in humans. The authors therefore conducted a phase I clinical trial to quantitatively detect T-cell responses induced by HIV-1 DNA vaccine in healthy individuals.