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目的探讨卵巢癌患者血清mi R-191含量,分析其与卵巢癌临床病理特征的关系。方法收集本院收治的上皮性卵巢癌患者血清样本50例,采用实时定量PCR方法检测血清中mi R-191的水平。同时收集卵巢癌患者的临床病理资料,分析mi R-191的含量与患者临床病理特征的关系。应用受试者工作曲线(ROC)分析mi R-191在卵巢癌诊断中的临床价值。选取同时期50例健康体检者作为对照。结果上皮性卵巢癌患者血清mi R-191的含量显著高于健康对照组,差异具有统计学意义(P<0.05)。手术-病理分期期别晚、有淋巴结转移者血清mi R-191含量显著高于手术-病理分期期别早和无淋巴结转移者(P<0.05)。血清mi R-191诊断卵巢癌的ROC曲线下面积(AUC)、灵敏度、特异度分别为0.85、84.7%、83%,95%可信区间(CI)为0.771~0.928。结论卵巢患者血清mi R-191水平升高,与患者临床病理特征有关。mi R-191在卵巢癌诊断中具有一定的价值,可应用于卵巢癌的辅助检查。
Objective To investigate the serum level of mi R-191 in patients with ovarian cancer and analyze its relationship with the clinicopathological features of ovarian cancer. Methods Fifty patients with epithelial ovarian cancer were collected from our hospital. The level of mi R-191 in serum was detected by real-time PCR. Clinicopathological data of ovarian cancer patients were also collected to analyze the relationship between the content of mi R-191 and the clinicopathological features of patients. Application of receiver operating curve (ROC) analysis of mi R-191 in the diagnosis of ovarian cancer clinical value. Select the same period of 50 cases of healthy subjects as a control. Results The serum level of mi R-191 in patients with epithelial ovarian cancer was significantly higher than that in healthy controls (P <0.05). The level of mi R-191 in patients with lymph node metastasis was significantly higher than those without lymph node metastasis at the stage of surgery-pathology (P <0.05). The area under the ROC curve (AUC), sensitivity and specificity of serum mi R-191 in diagnosis of ovarian cancer were 0.85, 84.7%, 83% and 95% confidence interval (CI) 0.771 to 0.928, respectively. Conclusion The serum level of mi R-191 in ovarian patients is elevated, which is related to the clinicopathological features of patients. mi R-191 in the diagnosis of ovarian cancer has a certain value, can be applied to the auxiliary examination of ovarian cancer.