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目的 探讨醛固酮合成酶基因CYP11B2在正常与肝纤维化大鼠肝组织中的表达。方法 48只雄性Wistar大鼠，随机分为模型组与对照组。模型组：40% CCl4 油2.5 ml/kg(b.w.皮下注射，每周3次；对照组：橄榄油2.5 ml/kg(b.w.皮下注射。于第4、6、8、10周处死大鼠，光镜下观察肝组织形态学变化。RT-PCR和原位杂交法检测CYP11B2 的表达。结果与结论 原位杂交法及RT-PCR检测显示CYP11B2在纤维化形成时表达增强，提示CYP11B2在纤维化肝脏的储脂细胞中表达增强。
Objective To investigate the expression of aldosterone synthase gene CYP11B2 in normal and hepatic fibrosis rats. Methods Forty-eight male Wistar rats were randomly divided into model group and control group. The model group: 40% CCl4 oil 2.5 ml / kg (bw subcutaneous injection, three times a week; control group: 2.5 ml / kg olive oil (bw subcutaneous injection. The morphological changes of liver tissue were observed by microscopy, and the expression of CYP11B2 was detected by RT-PCR and in situ hybridization.Results and Conclusion In situ hybridization and RT-PCR showed that the expression of CYP11B2 was enhanced during fibrosis, suggesting that CYP11B2 was expressed in fibrotic liver Of fat-storing cells.