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目的:定量评价紫杉醇对人大肠癌细胞的杀伤作用和药物敏感性.方法:用 M T T 方法检测紫杉醇对体外培养人大肠癌细胞 C C L?187、 C C L?229、 C X?1 和 CLONE A 的杀伤作用.结果:在最大生理耐受浓度(1*10－ 7M OL/ L)下,药物持续作用72 H,紫杉醇对4 种大肠癌细胞的生长抑制率 C C L?187 为 34?54％ , C C L?229 为 58?71％ , C X?1 为67?40％ , CLONE A 为70?87％ .其中对 C X?1 和 CLONE A 呈高度敏感性, I C50分别为9?0*10－ 10M OL/ L和4?0*10－ 9M OL/ L.紫杉醇对大肠癌细胞的杀伤作用随药物浓度和作用时间的增加而增加.结论:紫杉醇对人大肠癌细胞具有体外敏感性,对 C X?1 和 CLONE A 呈高度有效性.药物作用表现出明显的时间和剂量依赖性.“,”Objective:Quantitative evaluation of cytotoxicity and sensitivity of taxol against human colorectal cancer cell lines. Methods:MTT[3 (4,5 dimenthylthiazol z yl) 2,5 diphenyl tetrazolium bromide] assay was used to determine the inhibitory action of taxol on human colorectal cancer cells in vitro, CCL 187,CCL 229,CX 1 and Clone A. Results:At maximum physiologically tolerant concentration in plasam(1*10 -7 mol/L),taxol showed inhibitory activity on all four colorectal cancer cell lines, when cells were continuously exposed to taxol for 72 h. The inhibited growth rate was 34.54% for CCL 187, 58.71% for CCL 229, 67.40% for CX 1, and 70.87% for Clone A. Taxol showed strong cytotoxicity on CX 1 at IC 50 9.0*10 -10 mol/L and Clone A at IC 50 4.0*10 -9 mol/L,which is corresponding to the clinically effective concentration of taxol against several other tumor cells.Methods:MTT[3 (4,5 dimenthylthiazol z yl) 2,5 diphenyl tetrazolium bromide] assay was used to determine the inhibitory action of taxol on human colorectal cancer cells in vitro, CCL 187,CCL 229,CX 1 and Clone A. Results:At maximum physiologically tolerant concentration in plasam(1*10 -7 mol/L),taxol showed inhibitory activity on all four colorectal cancer cell lines, when cells were continuously exposed to taxol for 72 h. The inhibited growth rate was 34.54% for CCL 187, 58.71% for CCL 229, 67.40% for CX 1, and 70.87% for Clone A. Taxol showed strong cytotoxicity on CX 1 at IC 50 9.0*10 -10 mol/L and Clone A at IC 50 4.0*10 -9 mol/L,which is corresponding to the clinically effective concentration of taxol against several other tumor cells.MTT[3 (4,5 dimenthylthiazol z yl) 2,5 diphenyl tetrazolium bromide] assay was used to determine the inhibitory action of taxol on human colorectal cancer cells in vitro, CCL 187,CCL 229,CX 1 and Clone A. Results:At maximum physiologically tolerant concentration in plasam(1*10 -7 mol/L),taxol showed inhibitory activity on all four colorectal cancer cell lines, when cells were continuously exposed to taxol for 72 h. The inhibited growth rate was 34.54% for CCL 187, 58.71% for CCL 229, 67.40% for CX 1, and 70.87% for Clone A. Taxol showed strong cytotoxicity on CX 1 at IC 50 9.0*10 -10 mol/L and Clone A at IC 50 4.0*10 -9 mol/L,which is corresponding to the clinically effective concentration of taxol against several other tumor cells.Results:At maximum physiologically tolerant concentration in plasam(1*10 -7 mol/L),taxol showed inhibitory activity on all four colorectal cancer cell lines, when cells were continuously exposed to taxol for 72 h. The inhibited growth rate was 34.54% for CCL 187, 58.71% for CCL 229, 67.40% for CX 1, and 70.87% for Clone A. Taxol showed strong cytotoxicity on CX 1 at IC 50 9.0*10 -10 mol/L and Clone A at IC 50 4.0*10 -9 mol/L,which is corresponding to the clinically effective concentration of taxol against several other tumor cells.Conclusion:Taxol shown potent effect on human colorectal cancer cell lines in vitro,in particular on CX 1 and Clone A lines. The potency increased with the increasing concentration and duration of action for taxol.