论文部分内容阅读
研究了高粱雄性不育系11A与苏丹草3个杂交组合F2代的生长、育性、细胞遗传学特性及同工酶酶谱表型。结果表明:3种杂交组合F2代的生长势和平均株高均明显超过其各自父本苏丹草,生育期约140d,平均单株分蘖数5.3个,每组合分离出4类穗型,中间类型可作为后代优良单株选择的重要形态学依据;F2代花粉可育率在75.26%~86.05%之间,花粉不存在严重不育问题;F2代PMCM I的平均染色体构型均为2n=2x=20=10 II,与亲本相比,F2棒状二价体频率较高,约为50%,原亲本间的遗传组成存在着差异;F2代抽穗期4类穗型POD同工酶的酶带数、位点(R f)及强弱均存在一定差异,体现了基因在蛋白质表达水平上的差异,可作后代优良单株选择的遗传标记。
The growth, fertility, cytogenetic characteristics and isoenzyme zymogram phenotype of the F2 generation of sorghum CMS line 11A and sudangrass were studied. The results showed that the growth vigor and average plant height of F2 progenies of three cross combinations were significantly higher than that of their respective parents, Sudan grass. The average growth tillers per plant were about 140 days, and the average number of tillers per plant was 5.3. There were 4 types of panicles isolated per group, Which could be used as an important morphological basis for selection of excellent single plants in future generations. The fertile rate of pollen in F2 was between 75.26% and 86.05%, pollen did not have serious sterility problems. The average chromosome configuration of PMCM I in F2 was 2n = 2x = 20 = 10 II. Compared with the parent, the frequency of F2 rod-like bivalents was about 50%, and the genetic composition of the original parents was different. The bands of POD isozymes Number, locus (R f) and its strength were different, which reflected the difference in gene expression level of the gene and could be used as genetic markers for selection of excellent single plants in future generations.