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目的研究子痫前期患者血清和胎盘组织中VEGF的表达情况。方法血清VEGF测定采ELISA法,采用SP免疫组化法检测VEGF石蜡包埋的胎盘组织,切片,HE染色,光镜观察。将制好切片置显微镜下观察。结果判定:每张切片至少观察10个高倍视野,观测胎盘绒毛滋养细胞及间质细胞VEGF的表达。结果子痫前期患者血清和胎盘组织中VEGF的表达均明显的低于对照组(P<0.05),且与疾病的严重程度相一致。结论 VEGF可能通过直接或者间接途径影响滋养细胞的侵袭能力,参与子痫前期的发生发展,研究VEGF可能为子痫前期的早期诊断和治疗提供新的方法。
Objective To study the expression of VEGF in serum and placenta of patients with preeclampsia. Methods The serum levels of VEGF were determined by ELISA. SP immunohistochemistry was used to detect the expression of VEGF in paraffin embedded placenta. The sections were stained with HE and stained with light microscope. The system will be made into the microscope under the microscope. The results showed that: at least 10 high power fields were observed in each section, and the expression of VEGF in placental villous trophoblast and stromal cells was observed. Results The expression of VEGF in serum and placenta of preeclampsia patients was significantly lower than that of the control group (P <0.05), and was consistent with the severity of the disease. Conclusion VEGF may affect the invasive ability of trophoblast directly or indirectly and participate in the development of preeclampsia. It is suggested that VEGF may provide a new method for the early diagnosis and treatment of preeclampsia.