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用膜片钳技术首次研究了三氟氯氰菊酯对离体培养的棉铃虫中枢神经细胞延迟整流钾通道电流的影响。结果表明,药物作用前有81%和39%的细胞的通道分别在-30mV和-40mV激活(n=21)。三氟氯氰菊酯(10-5mmol/L)作用15min后,有63%和38%细胞的通道分别在-40mV和-50mV激活(n=8);作用1min后电流幅值明显降低,抑制率达到了37.7%(n=19);加药后激活曲线明显左移且Vh值变化显著,但k值没有明显变化。实验结果说明,三氟氯氰菊酯作用后,通道更容易激活,但显著抑制电流峰值,导致神经敏感性降低,棉铃虫中枢神经细胞钾通道也是拟除虫菊酯类药物的作用靶标之一。
Patch clamp technique was used to study the effect of cyhalothrin on the delayed rectifier potassium current in cultured central nervous system of cotton bollworm (Helicoverpa armigera). The results showed that 81% and 39% of the cells’ channels were activated at -30mV and -40mV (n = 21), respectively, before drug effect. After 15 minutes of cyhalothrin (10-5mmol / L), 63% and 38% of the cells were activated at -40mV and -50mV respectively (n = 8). After 1min, the amplitude of current decreased and the inhibition rate reached 37.7% (n = 19). After dosing, the activation curve obviously shifted to the left and the value of Vh changed significantly, but the value of k did not change significantly. The experimental results show that after cyhalothrin, the channel is more easily activated, but significantly inhibits the current peak, resulting in lower sensitivity of the nerve. The potassium channel in the central nervous cell of cotton bollworm is also one of the target of pyrethroid drugs.