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用细胞内微电极记录方法研究了重组人白细胞介素－１β（ｒｈＩＬ－１β）和重组人白细胞介素－２（ｒｈｉＬ－２）对分散培养的新生大鼠海马神经元膜电性质的影响。采用压力脉冲微量给药技术将白介素施加于所记录的细胞表面。结果发现：１００Ｕ／ｍｌ浓度的ｒｈＩＬ－１β使受作用的海马神经元超极化４．２０±１．８６ｍＶ；１００Ｕ／ｍｌ浓度的ｒｈＩＬ－２使５０％受作用的海马神经元去极化１１．１２±３．７１ｍＶ，并伴有强烈的自发放电反应，而１０００Ｕ／ｍｌ浓度的ｒｈＩＬ－２使１００％受作用的神经元超极化３．２５±０．６３ｍＶ，这些神经元的膜阻抗均无明显变化。本实验结果提示ｒｈＩＬ－１β和ｒｈＩＬ－２可显著影响体外培养海马神经元的膜兴奋性。
The effects of recombinant human interleukin-1β (rhIL-1β) and recombinant human interleukin-2 (rhiL-2) on the membrane electrical properties of hippocampal neurons in cultured neonatal rats were studied by intracellular microelectrode recording. Interleukin was applied to the recorded cell surface using a pressure pulse microdosing technique. As a result, it was found that rhIL-1β at a concentration of 100 U / ml hyperpolarized the affected hippocampal neurons by 4.20 ± 1.86 mV; rhIL-2 at a concentration of 100 U / ml depolarized 50% of the affected hippocampal neurons .12 ± 3.71 mV with a strong spontaneous discharge response while 1000 U / ml of rhIL-2 hyperpolarized 100% of the affected neurons 3.25 ± 0.63 mV, the membrane impedance of these neurons No significant changes. Our results suggest that rhIL-1β and rhIL-2 can significantly affect the membrane excitability of cultured hippocampal neurons in vitro.