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目的探讨自体吞噬相关基因Beclin 1和微管相关蛋白1轻链3(microtubule associated protein 1 light chain3,LC3)表达和腮腺肿瘤之间的关系。方法所用组织取自本院2001-2009年手术标本,用免疫组化法和免疫印迹法检测20例正常腮腺组织,37例腮腺多形性腺瘤,41例癌在多形性腺瘤中Beclin 1和LC3的表达,并结合临床病理因素进行分析。结果 Beclin 1、LC3在正常腮腺组织表达为(1.045±0.002)、(0.779±0.047),在腮腺多形性腺瘤中表达下降[(0.419±0.051)、(0.243±0.023),P<0.05],在癌在多形性腺瘤中二者阳性表达显著下降[(0.173±0.008)、(0.051±0.010),P<0.05]。淋巴结转移及TNM分期与Beclin 1的表达间有明显差异(P<0.05),不同侵袭性,不同淋巴结转移组与LC3的表达间有明显差异(P<0.05)。结论 Beclin 1和LC3在腮腺多形性腺瘤及癌在多形性腺瘤中表达下调,自体吞噬功能改变可能在腮腺肿瘤形成过程中具有一定作用。
Objective To investigate the relationship between autophagy-related genes Beclin 1 and microtubule associated protein 1 light chain3 (LC3) expression and parotid tumors. Methods Tissue was taken from our hospital from 2001 to 2009. Twenty normal parotid glands and 37 parotid pleomorphic adenomas were detected by immunohistochemistry and immunoblotting. Forty-one cases of Beclin 1 and LC3 expression, combined with clinical and pathological factors were analyzed. Results The expressions of Beclin 1 and LC3 in normal parotid gland were (1.045 ± 0.002) and (0.779 ± 0.047), respectively, which were significantly lower in parotid pleomorphic adenoma (0.419 ± 0.051 and 0.243 ± 0.023, P <0.05, respectively) The positive expression of the two in pleomorphic adenoma was significantly decreased ([0.173 ± 0.008], (0.051 ± 0.010), P <0.05]. Lymph node metastasis and TNM stage were significantly different from those of Beclin 1 (P <0.05). The expressions of LC3 in different invasive and different lymph node metastasis groups were significantly different (P <0.05). Conclusions Beclin 1 and LC3 are downregulated in pleomorphic adenoma and carcinoma in pleomorphic adenoma. Autophagy may play a role in the formation of parotid gland tumor.