经石蜡包埋的组织,用甲苯胺蓝染色显示肥大细胞的方法已得到了广泛的应用。作者用电镜制样过程中的树脂包埋块,行半薄切片,用甲苯胺蓝染色,光镜观察也能显示肥大细胞的特殊形态。另用石蜡切片,甲苯胺蓝染色光镜观察以及电镜观察证实了树脂包埋、半薄切片的观察结果。 材料及方法 取自外科手术切除的组织, The paraffin-embedded tissue, staining with toluidine blue mast cells has been widely used. The author used electron microscopy sample preparation resin embedding block, line semi-thin sections, with toluidine blue staining, light microscopy can also show the special morphology of mast cells. Another paraffin section, toluidine blue staining and electron microscopy confirmed the resin embedded, semi-thin section of the observation. Materials and methods were taken from surgically excised tissue,