By exploiting the cyclostationary property of non-cooperative source signals, the problem of joint time-difference-of-arrival (TDOA) and Doppler estimation is discussed, and the multi-cycle estimator is developed by generalizing the single-cycle estimator. Utilizing a special commercial FM radio as a non-cooperative transmitter, some practical experiments are carried out. The right estimates of TDOA and Doppler of a particular airplane can really be obtained by processing the measurements with the multi-cycle estimation algorithm. Through adding some slowly time-variant white Gaussian noise to the original measurements, some independent Monte Carlo simulations are performed, and the simulation results reveal that the multi-cycle estimator is really superior to the single-cycle estimator and the conventional cross-correlation method.