论文部分内容阅读
不断增殖是肿瘤的基本特征。一些基本的细胞周期调控分子参与了肿瘤的形成。细胞周期蛋白A(cyclinA)是在G1/S期表达的细胞周期蛋白 (cyclin) ,其异常高表达参与了肿瘤的形成。为了探讨cyclinA异常高表达与白血病细胞恶性增殖的关系 ,采用流式细胞仪胞浆内间接免疫荧光 /DNA双染色法半定量分析了急性白血病患者、门诊接受骨髓穿刺的患者、健康献血者这三种不同来源的外周血或骨髓标本细胞 ,以及白血病细胞系 (HL 6 0 )cyclinA表达水平。为了进一步研究cyclinA在白血病细胞增殖中的作用 ,用在体内、体外均有抗肿瘤作用的六亚甲基二乙酰胺 (HMBA)在体外抑制白血病细胞系HL 6 0增殖 ,并诱导其分化。增殖抑制效应采用四唑盐还原试验和细胞周期分析来检测。细胞表面分化抗原CD33、CD11b改变来检测分化。结果发现 ,两组来自白血病标本的细胞S期cyclinA (即急性白血病患者外周血或骨髓幼稚细胞和白血病细胞系 )表达阳性率要远远高于另两组 (门诊骨髓穿刺的患者骨髓细胞和健康献血员外周血细胞 ) ;在HMBA抗肿瘤细胞系HL 6 0增殖实验中发现随药物剂量的加大 ,增殖抑制和分化程度呈剂量依赖性 ;同时 ,胞内cyclinA蛋白表达水平被明显下调了 ,也呈剂量依赖性。结论 :白血病细胞S期cyclinA异常高表达 ,HMBA能下调S期cy
Proliferation is the basic feature of the tumor. Some basic cell cycle regulators are involved in tumor formation. Cyclin A is a cyclin expressed in the G1 / S phase. Abnormally high expression of cyclin A is involved in tumor formation. To investigate the relationship between abnormal cyclinA overexpression and malignant proliferation of leukemia cells, we analyzed the relationship between the abnormal expression of cyclinA and malignant proliferation of leukemia cells by semi-quantitative analysis by flow cytometry with indirect immunofluorescence / DNA double staining in cytoplasm of patients with acute leukemia, outpatients receiving bone marrow aspirate, and healthy blood donors A variety of different sources of peripheral blood or bone marrow specimens of cells, and leukemia cell line (HL 60) cyclinA expression levels. In order to further study the role of cyclinA in the proliferation of leukemia cells, HMBA, an anti-tumor drug in vivo and in vitro, inhibited the proliferation and induced the differentiation of leukemia cell line HL-60. Proliferation inhibition was measured using tetrazolium salt reduction assay and cell cycle analysis. Cell surface differentiation antigen CD33, CD11b change to detect differentiation. The results showed that positive rates of S-phase cyclinA (leukemia or bone marrow blasts and leukemia cell lines) in the two groups of leukemic cells were much higher than those in the other two groups (bone marrow cells and healthy patients in outpatient bone marrow biopsy Blood donors’ peripheral blood cells); in HMBA antitumor cell line HL 6 0 proliferation experiment, it was found that with the increase of the dosage of the drug, the inhibition of proliferation and the degree of differentiation were dose-dependent; at the same time, the expression of intracellular cyclin A protein was obviously down-regulated In a dose-dependent manner. Conclusion: S-phase cyclinA is highly expressed in leukemia cells, while HMBA can down-regulate S phase cy