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目的 :建立模拟在体血脑屏障 (blood brainbarrier ,BBB)形态、功能的体外细胞模型。方法 :将纯化的 1型星形胶质细胞、脑微血管内皮细胞分别接种于涂有鼠胶原的Transwell多聚酯膜两侧 ,共培养。分别检测共培养后BBB特异性酶γ 谷氨酰转酞酶 (γ GT)含量 ;测定荧光素钠 (FLU)、12 5Ⅰ 牛血清白蛋白 (BSA)在该模型上的通透量。结果 :共培养后内皮细胞表现出典型细胞形态 ;γ GT含量随共培养酶活性增强 ;模型对大分子蛋白质和小分子物质都具有良好限制通透的作用。结论 :脑微血管内皮细胞与 1型星形胶质细胞共培养能够形成与在体BBB类似的结构 ,并具有在体屏障的限制物质通透、酶屏障的功能
Objective: To establish an in vitro cell model that simulates the morphology and function of blood brain barrier (BBB). Methods: Purified type 1 astrocytes and brain microvascular endothelial cells were inoculated on both sides of Transwell multifilm coated with mouse collagen and co-cultured. The concentration of γ-GT of BBB-specific enzyme was detected after co-culture, and the permeation of fluorescein sodium (FLU) and 12 5-bovine serum albumin (BSA) were measured. Results: The endothelial cells showed typical cell morphology after co-culture. The γ GT content increased with the co-culture enzyme activity. The model had a good effect of limiting the permeation of macromolecules and small molecules. CONCLUSION: Co-culture of brain microvascular endothelial cells with type 1 astrocytes can form a structure similar to that of BBB in vivo and has the function of limiting substance permeability and enzyme barrier in the body barrier