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采用化学腐蚀法制备无细胞毒性的SiC量子点荧光材料,以硝酸及氢氟酸为腐蚀剂,通过超声分散及高速离心处理,获得碳化硅量子点水相溶液,对其微观组织、光学性能进行了检测,而后在出芽短梗霉菌(Aureobasidium pulluans)活体细胞上进行了荧光成像,结果表明,碳化硅量子点呈近球型,直径约为2.5nm,小于体材激子波尔直径(5.4nm),光致发光光谱证明了量子限制效应,激发光波长为320～360nm时,发射光强最大。激发光波长增加时,其Photo Luminescence(PL)峰发生红移,而当量子点直径减小时,会导致发射光蓝移。活体细胞荧光成像研究结果表明,该量子点无细胞毒性,对细胞生长生理机能几乎没有任何影响,可以实现活体细胞长时程的荧光成像。同时对其标记特征及原理进行了分析。
The non-cytotoxic SiC quantum dots fluorescent materials were prepared by chemical etching. The nitric acid and hydrofluoric acid were used as corrosive agents to obtain the aqueous solution of silicon carbide quantum dots by ultrasonic dispersion and high-speed centrifugation. The microstructure and optical properties (Aureobasidium pulluans) in vivo. The results show that the QDs are almost spherical and have a diameter of about 2.5 nm, which is smaller than the diameter of the exciton (5.4 nm) , Photoluminescence spectroscopy proved the quantum confinement effect, the excitation light wavelength of 320 ~ 360nm, the maximum emission intensity. When the wavelength of the excitation light increases, its Photo Luminescence (PL) peak is red-shifted, and when the diameter of the quantum dot is reduced, it causes a blue shift of the emitted light. Fluorescence imaging of living cells showed that this quantum dot had no cytotoxicity and had almost no effect on cell growth and physiological function, and could achieve long-term fluorescence imaging of living cells. At the same time, the characteristics and principles of the markings are analyzed.