论文部分内容阅读
2006-2008年,在浙江湖州开展了鳜(Siniperca chautsi)与斑鳜(Siniperca scherzeri)的种间杂交试验,并成功获得了鳜(♀)×斑鳜(♂)杂种F1。通过对杂种F1与其亲本的形态差异比较和微卫星标记分析,探讨了杂种F1的性状变异和遗传特征。结果表明:(1)7个可数性状中,杂种F1的背鳍条、腹鳍条和胸鳍条数与父母本一致,臀鳍条、鳃耙数与母本基本一致,侧线鳞、幽门盲囊数目介于父、母本之间;10个可量性状的平均杂种指数为39.08,显示杂种F1的可量性状略偏向母本;框架参数和可量性状的聚类分析、判别分析和主成份分析表明,杂种F1的体型介于父、母本之间,略倾向于母本,主要表现在躯干部和尾部的差异。(2)5对微卫星引物分析表明,杂种F1的等位基因均来源于双亲,杂交子代的遗传符合孟德尔遗传规律,属两性融合生殖,是真正意义上的杂交种。
In 2006-2008, interspecific hybridization between Siniperca chautsi and Siniperca scherzeri was carried out in Huzhou, Zhejiang Province, and hybrid F1 of 鳜 × 斑 ♂ was successfully obtained. Morphological variations and microsatellite markers of hybrid F1 were compared with those of their parents to investigate the genetic variation and genetic characteristics of F1 hybrids. The results showed that: (1) In seven countable traits, the numbers of dorsal, pelvic and pectoral fins of hybrid F1 were consistent with those of their parents. The number of gingival rachis and gill rachis was basically consistent with that of female parent. The average heterosis index of 10 measurable traits was 39.08, indicating that the measurable trait of F1 was slightly biased toward the female parent. The cluster analysis, discriminant analysis and principal component analysis of the frame parameters and measurable traits showed that, F1 hybrid body between father and mother, slightly inclined to the mother, mainly in the trunk and tail differences. (2) Five pairs of microsatellite primers showed that alleles of F1 hybrids originated from parents, and the inheritance of hybrid progeny was in accordance with Mendel ’s genetic rule. It belongs to sexual fusion and is a true hybrid.