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为了确定大珠母贝(Pinctada maxima)人工繁育过程中受精卵适宜孵化密度和不同发育期幼虫适宜培育密度,设立四组实验比较了培育密度对大珠母贝育苗效果的影响.实验Ⅰ,比较了受精卵培育密度对孵化率与幼虫大小的影响,受精卵密度设为10个/mL、20个/mL、30个/mL与40个/mL;实验Ⅱ,比较了D形幼虫培育密度对生长率与成活率的影响,幼虫密度设为1.0个/mL、2.0个/mL、3.0个/mL与4.0个/mL;实验Ⅲ,比较了壳顶初期幼虫培育密度对生长率与成活率的影响,幼虫密度为1.0个/mL、1.5个/mL、2.0个/mL与2.5个/mL;实验Ⅳ,比较了眼点期幼虫密度对变态率与生长率的影响,眼点幼虫密度设为0.4个/mL、0.8个/mL、1.2个/mL与1.6个/mL.结果表明,大珠母贝受精卵培育密度对孵化率存在显著影响(P＜0.05),对幼虫大小影响不显著(P＞0.05).培育密度对D形幼虫与壳顶幼虫的生长率和成活率均存在显著影响(P＜0.05),生长率与成活率随着密度增加而降低.培育密度对眼点幼虫的变态率和附着幼苗的生长存在显著影响(P＜0.05).大珠母贝苗种繁育阶段,合适的受精卵培育密度为10-30个/mL,D形幼虫培育密度为1-3个/mL,壳顶初期幼虫为1.0-1.5个/mL,眼点幼虫为0.4-0.8个/mL.“,”In order to determine the appropriate stocking density of egg and larvae of pearl oyster Pinctada maxima at different developmental stages during hatchery production,four experiments were carried out to investigate the effects of stocking density on early performances of pearl oyster Pinctada maxima.In Exp Ⅰ,the effects of fertilized egg density on hatching rate and growth of D-shaped larvae were investigated.The densities were set at 10,20,30 and 40 eggs/mL.In Exp Ⅱ,the effects of D-shaped larvae density on growth and survival rate were investigated.The densities were set at 1.0,2.0,3.0 and 4.0 larvae/mL.In Exp Ⅲ,the effects of umbone larvae density on growth and survival rate were investigated.The densities were set at 1.0,1.5,2.0 and 2.5 larvae/mL.In Exp Ⅳ,the effects of eye-spotted larvae density on growth and metamorphosis rate were investigated.The densities were set at 0.4,0.8,1.2 and 1.6 larvae/mL.The results showed that effects of densities of fertilized egg on hatching rate (P＜0.05) were significant.However,the effects of densities of fertilized egg on growth of D-shaped larvae were not significant (P＞0.05).The effects of stocking density on growth and survival rate of D-shaped and umbone larvae were significant (P＜0.05).Growth and survival rate of D-shaped and umbone larvae decreased with increasing density.The effects of stocking density on growth and metamorphosis rate of eye-spotted larvae were significant (P＜0.05).The present results suggest that the optimal densities of fertilized egg,D-shaped,early umbone and eye-spotted larvae should be 10-30 eggs/mL,1.0-3.0,1.0-1.5 and 0.4-0.8 larvae/mL,respectively,in the hatchery production of pearl oyster Pinctada maxima.