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用经过筛选的１１ 个籼粳分化特异探针对培矮６４Ｓ、Ｎ４２２Ｓ、１０８Ｓ、ＬＳ２Ｓ等４ 个不育系和５７ 份恢复系的ＤＮＡ 籼粳分化程度进行了初步研究。结果表明，供试材料ＤＮＡ 在籼粳分化程度上存在明显差异，表现在其ＴＤｊ值的分布范围为０．０６７４～０．８８６４。大多数品种籼粳分化比较彻底，ＤＮＡ 上处于典型籼粳之间的中间型的材料很少。培矮６４Ｓ、中４１３、特青２ 号、桂朝２号、晚三、密阳２３ 等品种的ＴＤｊ值小于０．１，属于典型籼稻；优良三系杂交籼稻恢复系明恢６３ 和密阳４６ 虽然在分类上属于籼稻，但都含有一定粳稻成分。ＬＳ２Ｓ、零轮、Ｃ４１８２、Ｃ４１８、Ｃ４１１、中作９２９９、中作９４６、中花３ 号、Ｍａｒｓ等都为偏粳，它们的ＴＤｊ值均小于秋光、中作９３ 等典型粳稻。通过部分品种的ＤＮＡ 的ＴＤｊ值与同工酶ＴＤｊ值比较研究发现，二者结果基本一致，但ＤＮＡ ＴＤｊ值提高了检测不同品种遗传分化程度的灵敏度。
A total of 11 indica / japonica differentiation-specific probes were selected to screen the indica-japonica differentiation of 4 CMS lines and 57 restorer lines of Pei’ai 64S, N422S, 108S and LS2S. The results showed that there was a significant difference in the degree of indica-japonica differentiation of the tested materials, which showed that the TDj value ranged from 0.0674 to 0.8864. Most varieties of indica and japonica differentiation more complete, DNA in the middle of typical indica and japonica between the material is very small. The TDj values of Pei’ai 64S, Zhong 413, Teqing 2, Gui Chai 2, late 3 and Mieyang 23 were less than 0.1, belonging to typical indica rice. The elite indica rice restorer lines Minghui63 and Miaoyang 46 Although they belong to indica rice in the classification, they all contain some japonica components. LS2S, zero round, C4182, C418, C411, made in 9299, made in 946, Zhonghua 3, Mars and so on are partial Japonica, their TDj values are less than autumn light, for 93 and other typical japonica rice. Through the comparison of the TDj value and the TDj value of some varieties of DNA, the results of the two methods are basically the same, but the DNA TDj value increases the sensitivity of detecting the degree of genetic differentiation of different varieties.