论文部分内容阅读
目的 研究抗HIV感染者淋巴细胞HIV 1辅受体突变的情况 ,阐明其感染抑制的分子机理。方法 设计特定的引物 ,用PCR方法检测健康人、HIV感染者和抗HIV感染者的基因组DNA中HIV辅受体 (CCR5 )是否突变。结果 抗HIV感染者发生CCR5△ 32纯合子突变 ,健康人及HIV感染者CCR5基因未发生突变。讨论 发现了我国本土人群存在CCR5△ 32纯合子突变 ,此突变可能为抗HIV感染保护机制 ,为艾滋病的治疗及预防提供新的方法和途径
Objective To study the mutation of HIV 1 co-receptor in lymphocytes of HIV-infected individuals and elucidate the molecular mechanism of infection inhibition. Methods Specific primers were designed and used to detect the presence or absence of HIV co-receptor (CCR5) mutations in genomic DNA of healthy, HIV-infected, and HIV-infected individuals by PCR. Results CCR5 △ 32 homozygous mutation occurred in HIV-infected persons, and CCR5 gene was not changed in healthy people and HIV-infected persons. The discussion found that CCR5 △ 32 homozygote mutation exists in the local population in our country, and this mutation may be the mechanism of protection against HIV infection and provide new methods and approaches for the treatment and prevention of AIDS