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目的 :研究食管癌及食管上皮人类白细胞抗原 (HLA A ,B ,C)的抗原表达及探索该抗原丢失的分子机理。方法 :采用抗HLA A ,B ,C单抗 ,以免疫组化法及甲基化PCR检测HLA A ,B ,C抗原在食管癌及食管上皮的分布及HLA A ,B ,C基因甲基化状况。结果 :31例食管癌中 2 7例无HLA A ,B ,C抗原表达 ,正常及异常增生的食管上皮均可见该抗原的阳性表达 ;45 2 %的食管癌HLA A ,B ,C基因甲基化 ,食管上皮该基因无甲基化。结论 :食管癌中HLA A ,B ,C抗原大部分丢失 ,基因甲基化可能是导致抗原下调的关键机理
Objective: To study the antigen expression of human leukocyte antigen (HLA A, B, C) in esophageal cancer and esophageal epithelium and to explore the molecular mechanism of this antigen loss. METHODS: The distribution of HLA A, B and C antigens in esophageal cancer and esophageal epithelium and the methylation of HLA A, B and C genes were detected by immunohistochemistry and methylation PCR using anti-HLA A, B and C monoclonal antibodies. situation. Results: Of the 31 cases of esophageal cancer, 27 cases of esophageal epithelium without HLA A, B, C antigen expression, normal and abnormal hyperplasia showed positive expression of the antigen; 45 2% of esophageal cancer HLA A, B, C gene methyl The gene is not methylated in esophageal epithelium. Conclusion : Most of the HLA A, B and C antigens were lost in esophageal cancer. Gene methylation may be the key mechanism leading to antigen downregulation.