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目的 探讨输血传播病毒 (transfusiontransmittedvirus,TTV)在人肝组织中的分布状况及其意义。方法 采用地高辛标记探针 ,对肝组织中的TTV基因进行原位杂交定位检测。结果 原位杂交染色阳性者为细胞核团块状。 5例正常人中有 1例阳性 ,5例乙型肝炎患者有 3例阳性 ,4例丙型肝炎患者有 2例阳性 ,3例戊型肝炎患者有 1例阳性。结论 TTV可感染肝细胞并在肝细胞核内复制。正常人及不同型肝炎病人的肝细胞内均可感染TTV。
Objective To investigate the distribution of transfusion transmitted virus (TTV) in human liver and its significance. Methods Digoxigenin-labeled probe was used to detect the TTV gene in liver tissue by in situ hybridization. Results In situ hybridization positive for the nucleus mass. One of five normal individuals was positive, three were positive in five hepatitis B patients, two were positive in four hepatitis C patients, and one was positive in three hepatitis E patients. Conclusion TTV can infect hepatocytes and replicate in the nucleus of hepatocytes. TTV can be infected in the liver cells of normal people and patients with different types of hepatitis.