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本文提出在KOH介质中,KIO_4—H_2O_2协同氧化化学发光测定牛血红蛋白(Hb)分析法。线性范围为1.0×10~(-5)-1.6×10~(-10)g/mL。初步探讨了Hb的化学发光机理。本法用于牛血中Hb的直接测定,获得满意结果。
In this paper, KIO_4-H_2O_2 synergistic oxidative chemiluminescence was used to determine the activity of bovine hemoglobin (Hb) in KOH medium. The linear range was 1.0 × 10 -5 -1.6 × 10 -10 g / mL. The chemiluminescence mechanism of Hb was preliminarily discussed. This method is used for the direct determination of Hb in bovine blood, to obtain satisfactory results.