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应用逆转录 聚合酶链反应 (RT PCR)对不同类型白血病患者骨髓 (BM )或外周血 (PB)细胞GATA 2和IgH胚系基因Cμ mRNA的表达进行检测 ,探讨GATA 2与IgH胚系基因Cμ 在白血病细胞中的表达与共表达及意义。在正常人BM和PB细胞中未检测到GATA 2和IgH胚系基因Cμ。在急性髓性白血病 (AML)和急性淋巴细胞白血病 (ALL)以及慢性粒细胞白血病慢性期(CML CP)GATA 2检出率分别为 91.3% ,75 %和 83.3% ,而IgH胚系基因Cμ 检出率分别为4 7.8% ,4 1.6%和 5 .6% ,慢性粒细胞白血病加速期 (AP)和急变期 (BC)全部表达GATA 2 ,其中部分病例表达IgH胚系基因Cμ。 4 7.8%的AML ,4 1.6%的ALL ,5 .6%的CML CP和 33.3%的CML AP及 4 0 %的CML BC患者共表达GATA 2和IgH胚系基因Cμ。GATA 2 +IgH胚系基因Cμ+AML和ALL细胞表面标志以HLA DR的表达为主。GATA 2作为造血系统的转录调节因子在白血病中的异常高表达是一个常见现象 ,胚系基因的活化与白血病的发生密切相关 ,是白血病发生机制中的另一重要环节。GATA 2与IgH胚系基因Cμ 的共表达提示白血病的发生是多种机制共同作用的结果。GATA 2 +IgH胚系基因Cμ+白血病 (包括AML ,ALL ,CLL)以HLA DR的表达占优势 ,推测其起源在早期造血干 祖细胞水平
The mRNA expression of GATA 2 and IgH in germline gene of bone marrow (BM) or peripheral blood (PB) of different types of leukemia patients were detected by reverse transcriptase-polymerase chain reaction (RT PCR), and the relationship between GATA 2 and IgH germline gene Cμ In leukemia cells in the expression and co-expression and significance. GATA 2 and IgH germline genes Cμ were not detected in normal human BM and PB cells. The detection rates of GATA 2 in acute myelogenous leukemia (AML), acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML CP) were 91.3%, 75% and 83.3%, respectively, whereas the IgH germline gene Cμ The rates were 4 7.8%, 41.6% and 5.6% respectively. Both GAP-2 and GAP-2 were expressed in the AP and BC of chronic myeloid leukemia, and some cases expressed IgM germline Cμ. 4 7.8% of AML, 4 1.6% of ALL, 5.6% of CML CP and 33.3% of CML AP and 40% of CML BC patients co-express GATA 2 and IgH germline genes Cμ. GATA 2 + IgH germline gene Cμ + AML and ALL cell surface markers HLA DR expression based. The abnormal expression of GATA 2 as a transcriptional regulator of hematopoietic system in leukemia is a common phenomenon. The activation of germline genes is closely related to the occurrence of leukemia and is another important link in the pathogenesis of leukemia. The co-expression of Cμ in GATA 2 and IgH germline genes suggests that the pathogenesis of leukemia is the result of a combination of mechanisms. GATA 2 + IgH germline Cμ + leukemia (including AML, ALL, CLL) HLA DR expression predominates, suggesting that its origin in the early hematopoietic stem and progenitor cell levels