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目的 通过比较在超声波热疗时不同的热剂量对荷人肝癌 (L C)裸鼠的治疗作用 ,探讨有效热剂量在超声热疗中的重要性。方法 以频率为 3.5 MHz的超声波照射荷人肝癌 (L C)裸鼠 ,取不同的设定温度和持续时间 ,每日测量肿瘤的体积并绘制体积 -时间曲线。两周后处死动物 ,肿瘤标本作病理学检查。结果 和对照组比较 ,治疗剂量组 (43℃ ,30分钟 )肿瘤体积从照射后的第三天起呈下降趋势 ,光镜下可见癌细胞排列紊乱 ,伴大量凝固性坏死 ;而小剂量组 (41℃ ,12分钟 )肿瘤体积呈上升趋势 ,光镜下见癌细胞巢状分布 ,癌巢间纤维组织增生。结论 热剂量在超声波热疗中应被看作是一极其关键的因素 ,热剂量过小反而可促进肿瘤的生长。
Objective To explore the importance of effective thermal dose in ultrasound hyperthermia by comparing the therapeutic effects of different thermal doses on human liver cancer (L C) nude mice during hyperthermia. Methods Human adult hepatocellular carcinoma (L C) nude mice were irradiated with ultrasound with a frequency of 3.5 MHz. Different temperature and duration were set. The volume of the tumor was measured daily and the volume-time curve was plotted. Two weeks later, animals were sacrificed and tumor specimens were examined for pathology. Results Compared with the control group, the tumor volume in the treatment group (43°C, 30 minutes) showed a decreasing trend from the third day after irradiation. Under the light microscope, the cancer cells were arranged in disorder with a large number of coagulative necrosis; while the small dose group ( At 41°C, 12 minutes, the tumor volume showed an upward trend. Under the light microscope, the cancerous cells showed a nested distribution and the fibrous tissue between the cancer nests was hyperplastic. Conclusion Thermal dose should be regarded as an extremely critical factor in ultrasound hyperthermia. If the thermal dose is too small, tumor growth can be promoted.