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香蕉与枯萎病互作机理研究一直是近年来研究的热点,目前对枯萎病菌的致病机理及香蕉的感、抗病机理还不清楚。本文利用前期表达谱分析结果克隆了乙烯合成及调控途径中的关键酶基因乙烯形成酶基因(MaACO)和乙烯响应因子基因(MaERF1),通过RT-PCR检测,对这两个基因在感、抗病香蕉种质中的表达水平进行研究。结果表明,MaACO和MaERF1基因对机械损伤非常敏感,尤其在损伤初期(3h)的表达水平远高于后期;对于枯萎病菌侵染处理的植株,尤其在感染初期(3h)MaACO和MaERF1的表达水平在抗性植株中的表达量均比感病植株中的低;抗性种质中乙烯途径可能受到抑制,香蕉的感病性可能与侵染初期对乙烯信号的敏感性相关。本研究为利用乙烯抑制剂进行香蕉抗枯萎病新方法的研究奠定了基础。
The interaction mechanism between banana and Fusarium wilt has been a hot spot in recent years. At present, the pathogenic mechanism of Fusarium wilt and the sense of banana and the mechanism of disease resistance are not clear. In this paper, the genes of ethylene-forming factor (MaACO) and ethylene-responsive factor (MaERF1), which are the key enzyme genes in ethylene synthesis and regulation pathway, were cloned by using expression profiling in the early stage. Disease banana germplasm expression levels were studied. The results showed that MaACO and MaERF1 genes were very sensitive to mechanical injury, especially at the early stage of injury (3h). The expression levels of MaACO and MaERF1 in plants infected by Fusarium oxysporum, especially at the early stage of infection (3h) The expression level of ethylene in resistant plants was lower than that in susceptible plants. The ethylene pathway in resistant germplasm might be inhibited. The susceptibility of banana may be related to the sensitivity to ethylene signal in the early stage of infection. This study laid the foundation for the new method of banana wilt resistance using ethylene inhibitors.