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目的探讨扶正解毒含药血清对染镍人支气管上皮细胞(16HBE)表达P38丝裂原活化蛋白激酶的影响,阐述镍化合物致癌的分子机制。方法用生理盐水,硫化镍(NiS),NiS+扶正解毒含药血清(低、中、高)剂量来处理16HBE后,用Western-blot法观察磷酸化P38MAPK的表达,处理细胞后提取蛋白,电泳、转膜、封闭、杂交、显影、洗脱。结果硫化镍能明显激活16HBE磷酸化P38MAPK的表达,硫化镍浓度越高,磷酸化P38MAPK的表达值越大,且存在剂量-效应关系,扶正解毒含药血清能明显抑制磷酸化P38MAPK的表达,尤以中、高剂量扶正解毒含药血清为甚,NiS+扶正解毒含药血清(中、高)剂量组与NiS比较抑制了磷酸化P38MAPK的表达(P<0.01)。结论硫化镍能明显激活磷酸化P38MAPK的表达,这可能是镍致癌的分子机制之一;扶正解毒含药血清能明显抑制16HBE磷酸化P38MAPK的表达,对于镍化合物诱发肿瘤的预防及治疗具有重要的意义。
Objective To investigate the effect of Fuzheng Jiedu drug-containing serum on the expression of P38 mitogen-activated protein kinase in nickel-labeled human bronchial epithelial cells (16HBE) and to elucidate the molecular mechanism of nickel compound carcinogenesis. Methods After treatment of 16HBE with saline, NiS and NiS + Fuzheng detoxified serum (low, medium and high), the expression of phosphorylated P38MAPK was observed by Western-blot and the proteins were extracted and electrophoresed, Transfection, blocking, hybridization, development, elution. Results Nickel sulphide significantly activated the expression of phosphorylated P38MAPK in 16HBE. The higher the concentration of nickel sulphide, the higher the expression of phosphorylated P38MAPK and the dose-effect relationship. The serum of Fuzhengjiedu Decoction could obviously inhibit the expression of phosphorylated P38MAPK, especially The middle and high dose of Fuzheng detoxification serum was more than that of NiS + Fuzheng detoxified serum (middle dose and high dose), which inhibited the phosphorylation of P38MAPK (P <0.01). Conclusion Nickel sulphide can activate the expression of phosphorylated P38MAPK, which may be one of the molecular mechanisms of carcinogenesis of nickel. Fuzheng detoxified serum can significantly inhibit the phosphorylation of P38MAPK by 16HBE, which is important for the prevention and treatment of nickel-induced tumors significance.