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【目的】研究聚乙二醇作为佐剂是否能够增强HBVDNA疫苗体液和细胞免疫效果。【方法】我们将乙型肝炎病毒(HBV)DNA疫苗(pVAX-S2)单独或PEG/pVAX-S2免疫C57BL/6J小鼠,在最后一次免疫14d检测抗-HBsIgG、T淋巴细胞增殖、细胞因子表达及体内细胞毒T淋巴细胞杀伤作用(CTL)等免疫学指标。【结果】结果表明,与仅免疫pVAX-S2比,PEG/pVAX-S2能够增加免疫小鼠抗-HBsIgG水平;混合免疫组的T淋巴细胞体外经乙肝表面抗原(HBsAg)刺激后,T淋巴细胞增殖数量显著高于对照组;另外,混合免疫组CD4+T淋巴细胞中IL-4和IFN-γ以及CD8+T淋巴细胞中IFN-γ的表达显著高于对照组。更重要的是,混和免疫组HBsAg特异性CTL显著高于对照组。【结论】总之,我们首次证明PEG作为佐剂不仅能够增强体液免疫反应和细胞免疫反应,而且能够显著增强体内CTL的杀伤活性,为HBV DNA的进一步研究奠定了基础。
【Objective】 To investigate whether polyethylene glycol (PEG) can enhance the humoral and cellular immunity of HBVDNA vaccine as an adjuvant. 【Methods】 C57BL / 6J mice were immunized with HBV DNA vaccine (pVAX-S2) alone or PEG / pVAX-S2, and anti-HBs IgG, T lymphocyte proliferation and cytokines were detected 14 days after the last immunization Expression and cytotoxic T lymphocyte cytotoxicity (CTL) and other immunological indicators. 【Results】 The results showed that PEG / pVAX-S2 could increase the level of anti-HBs IgG in immunized mice compared with pVAX-S2 alone. T lymphocytes The number of proliferating cells was significantly higher than that of the control group. In addition, the expression of IFN-γ in IL-4 and IFN-γ and CD8 + T lymphocytes of CD4 + T lymphocytes in mixed immunized group was significantly higher than that in control group. More importantly, HBsAg-specific CTL in the mixed immunization group was significantly higher than that in the control group. [Conclusion] In conclusion, we have demonstrated for the first time that PEG as an adjuvant can not only enhance humoral and cellular immune responses, but also significantly enhance CTL cytotoxic activity in vivo, laying a foundation for further study of HBV DNA.