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本研究以硝酸盐营养缺陷型突变体(nit)和多菌灵抗性为遗传标记,在5个所选菌株中设计了3个玉蜀黍赤霉病菌Gibberella zeae的杂交组合,使各菌株之间进行杂交,从而诱导有性重组体。从各杂交组合的后代中任意挑选出3个有性重组体,比较了这些有性重组体与其亲本在无性和有性阶段的主要生物学性状。结果表明,玉蜀黍赤霉中的nit基因及对杀菌剂多菌灵的抗药性基因可以通过有性杂交的方式重组,即发生了有性重组。有性重组体与其亲本在菌落生长、培养性状和致病性方面没有显著差异;但某些有性重组体中,产孢量和产子囊壳能力方面存在一定差异。总体看来,有性重组体仍然保持了较高的适合度。因此,可以认为有性重组在玉蜀黍赤霉群体对多菌灵抗药性发生发展以及群体遗传进化中可能起着重要的作用。
In this study, nitrate-auxotrophic (nitr) and carbendazim resistance were used as genetic markers. Three crosses of Gibberella zeae were designed among the five selected strains, Hybridization, which induces sexual recombinants. Three sexual recombinants were randomly selected from the descendants of each crosses, and the main biological traits of these sexual recombinants and their parents in the sexual and sexual stages were compared. The results showed that the nit gene in Gibberella zeae and the multidrug-resistant bactericide carbendazim could be recombined by sexual hybridization, that is, sexual recombination occurred. Sex recombinants and their parents did not differ significantly in colony growth, culture and pathogenicity, but in some sexual recombinants there were some differences in sporulation and capsular capacity. In general, sexual recombination still maintained a high degree of fitness. Therefore, it can be considered that sexual recombination may play an important role in the development of the resistance of carbendazim and the population genetic evolution in Zea mays population.