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高血压是一种慢性血管性疾病,易累及肾、肝、心、脑等组织,引起脑卒中和心、肾损害等并发症。本研究对高血压时肾、肝、心、脑等组织的炎症状态进行了观察。实验采用自发性高血压大鼠(spontaneously hypertensive rat,SHR)和正常血压的Wistar-Kyoto(WKY)大鼠,用RT-PCR和Western blot法观察肾、肝、心、脑等组织炎症相关因子IL-1β、TNFα、ICAM-1、iNOS、C/EBPδ和PPARγ的基因表达：紫外分光光度法观察蛋白质羰基化水平和FRAP法检测组织总抗氧化能力。结果显示：(1)SHR组织炎症相关因子表达较对照WKY增强,除IL-1βmRNA在肝和脑的增加不明显外,其余均有显著性差异(P＜0．05)；(2)SHR和WKY大鼠肾、心、脑蛋白质羰基化水平(nmol/mg蛋白)分别为8．93±1．08和2．27±0．43、2．23±0．23和0．17±0．02、13．42±1．10和5．72±1．01,SHR明显增加(P＜0．05)；而肝脏蛋白质羰基化水平无明显变化：(3)SHR肾、肝、心、脑总抗氧化能力水平显著低于WKY大鼠(P＜0．05)。以上结果表明,SHR多个组织(肾、肝、心和脑)均存在炎症因子被诱导和氧化应激反应等明显的炎症状态,提示炎症可能在高血压及其并发症的病理改变中起重要作用。
Hypertension is a chronic vascular disease that easily affects the kidneys, liver, heart, brain and other tissues and causes complications such as stroke and heart and kidney damage. The study of hypertension, renal, liver, heart, brain and other tissues of the inflammatory state were observed. The Wistar-Kyoto (WKY) rats with spontaneous hypertensive rats (SHR) and normal blood pressure were used in this study. The expression of inflammatory cytokines IL, IL- 1β, TNFα, ICAM-1, iNOS, C / EBPδ and PPARγ were measured by enzyme-linked immunosorbent assay (ELISA). The carbonylation level of protein was measured by UV spectrophotometry. The results showed that: (1) Compared with WKY, the expression of inflammation-associated factor in SHR tissues was increased, except for the obvious increase in IL-1β mRNA in the liver and brain (P <0.05); (2) WKY rats kidney, heart and brain protein levels of carbonylation (nmol / mg protein) were 8.93 ± 1.08 and 2.27 ± 0.43, 2.23 ± 0.23 and 0.17 ± 0. 02, 13.42 ± 1.10 and 5.72 ± 1.01, SHR was significantly increased (P <0.05), while there was no significant change in hepatic protein carbonylation level: (3) SHR kidney, liver, heart and brain Total antioxidant capacity was significantly lower in WKY rats (P <0.05). The above results show that there are obvious inflammation states such as inflammatory factor induction and oxidative stress in multiple tissues of SHR (kidney, liver, heart and brain), suggesting that inflammation may play an important role in the pathological changes of hypertension and its complications effect.