目的:探讨中药“乙肝散”抗免疫性肝纤维化的作用,为临床应用乙肝散抗肝纤维化提供了理论依据。方法:雄性Wistar大鼠尾静脉注射白蛋白建立实验性免疫性肝纤维化模型,分组加用不同浓度的乙肝散颗粒饲料,实验结束宰杀动物,检测血清HA、AST、ALT、ALB指标以及肝组织病理、肝组织化学染色、Ⅳ胶原免疫组化、a-SMA免疫组化、肝匀浆Hyp、和流式细胞仪a-SMA阳性细胞定量分析。结果:高浓度组(B组)、低浓度组(C组)和正常对照组(D组)血清AST、ALT和肝组织匀浆Hyp水平,a-SMA阳性细胞定量分析,B组和D组血清HA均明显低于模型组(A组)(P<0.01),A组大鼠肝组织肝细胞普遍变性坏死,汇管区大量胶原纤维、网状纤维和Ⅳ胶原沉积,相互连接形成纤维隔,重新分割肝小叶,部分假小叶形成。B组和C组肝细胞坏死及胶原纤维、网状纤维和Ⅳ胶原纤维组织增生程度明显减轻。结论:中药乙抑制HSC活化.抑制胶原纤维、网状纤维、Ⅳ型胶原的合成,具有抗免疫性肝纤维化作用。 Objective: To investigate the anti-immune hepatic fibrosis effect of “Yi-Gan Powder” and provide a theoretical basis for the clinical application of Yi Gan San in the treatment of hepatic fibrosis. METHODS: Male Wistar rats were injected with albumin via tail vein to establish an experimental hepatic fibrosis model. The rats were divided into two groups with different concentrations of Yigan San granules. Animals were slaughtered at the end of the experiment to detect serum HA, AST, ALT, ALB indexes and liver tissues. Pathology, hepatic histochemical staining, IV collagen immunohistochemistry, a-SMA immunohistochemistry, liver homogenate Hyp, and flow cytometry a-SMA positive cell quantitative analysis. Results: Serum AST, ALT and liver homogenate Hyp levels in high-concentration group (group B), low-concentration group (group C) and normal control group (group D), quantitative analysis of a-SMA positive cells, group B and group D Serum HA was significantly lower than model group (A) (P<0.01). Liver cells of group A were degenerate and necrosis, and a large number of collagen fibers, reticular fibers, and collagen IV were deposited in the portal area and they were connected to form a fibrous septum. The liver lobes were re-divided and part of the pseudo-lobules formed. The necrosis of hepatocytes and the proliferation of collagen fibers, reticular fibers and IV collagen fibers were significantly reduced in groups B and C. Conclusion: Chinese medicine B can inhibit the activation of HSC. It inhibits the synthesis of collagen fibers, reticular fibers, and type IV collagen and has anti-immune liver fibrosis effect.