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目的观察γ-干扰素对猪血清诱导的大鼠免疫性肝纤维化的抑制作用。方法用猪血清腹腔注射复制免疫损伤性肝纤维化模型,9周后给大鼠每天肌肉注射γ-干扰素10万国际标准单位,共21天,以正常和模型组作为对照,第12周处死动物,测定体重、HA、LN、PCⅢ、Ⅳ-C并进行肝纤维化分级。结果γ-干扰素治疗组大鼠的肝纤维化生化指标HA、LN、PCⅢ、Ⅳ-C放免测定值较模型组明显下降(P<0.01)而接近正常对照组(P>0.05),肝纤维化的分级程度较模型组减轻(P<0.01),大鼠体重较正常组和模型组稍有下降(P>0.05)。结论γ-干扰素能有效抑制猪血清诱导的大鼠免疫性肝纤维化,其作用表现为IFN-γ不仅能够抑制胶原纤维进一步形成,更有强劲的促进胶原降解的作用。
Objective To observe the inhibitory effect of IFN-γ on pig serum-induced immune liver fibrosis in rats. Methods The model of immunocompromised liver fibrosis was injected intraperitoneally with porcine serum. After 9 weeks, the rats were intramuscularly injected with 100,000 international standard units of interferon gamma (IFN-γ) for 21 days. The rats in the normal and model groups were sacrificed at the 12th week Animals, weight, HA, LN, PCⅢ, Ⅳ-C and liver fibrosis grading. Results The radioimmunoassay of HA, LN, PCⅢ and Ⅳ-C in liver of IFN-γ-treated rats was significantly lower than that of the model group (P <0.01), and close to the normal control group (P> 0.05) (P <0.01). The body weight of the rats decreased slightly compared with the normal group and the model group (P> 0.05). Conclusions γ-interferon can effectively inhibit pig serum-induced immune-induced hepatic fibrosis in rats. The effect of IFN-γ is not only able to inhibit the further formation of collagen fibers, but also promote collagen degradation more strongly.