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目的探讨微小RNA542-3p(miR-542-3p)在结直肠癌患者癌组织中的表达情况。方法选取40例结直肠癌患者的癌组织、配对远癌正常肠上皮组织、人正常肠上皮细胞株HIEC和结直肠癌细胞株Lovo,LS1747,SW620,HT29,SW480为研究对象。用实时荧光聚合酶链式反应法检测miR-542-3p在结直肠癌组织、正常肠上皮组织、肠癌细胞及正常肠上皮细胞株中的表达水平。通过外源转染miR-542-3p mimics或inhibitor,分别于转染后24,48,72,96 h检测HT29细胞的增殖能力;用划痕实验检测转染前后HT29细胞迁移能力评价miR-542-3p在肠癌细胞中的生物学功能。结果 MiR-542-3p表达在癌组织中显著低于正常肠上皮组织(P<0.05);miR-542-3p在结直肠癌细胞株HT29,SW620,Lovo中表达较正常肠细胞株显著降低(P<0.05),而在配对细胞株中,在高转移能力的结直肠癌细胞株SW620及Lovo中表达同样显著降低(P<0.05)。24,48,72,96 h后,miR-542-3p inhibitor组、miR-542-3p mimics组、对照组及mimics control组的细胞增殖能力比较差异无统计学意义(P>0.05)。24 h后,miR-542-3p mimics组迁移能力显著低于mimics control组(P<0.05),而miR-542-3p inhibitor组迁移能力显著高于inhibitor control组(P<0.05)。结论 MiR-542-3p与结直肠癌细胞的侵袭转移能力有关,可作为肠癌侵袭转移的分子标志物。
Objective To investigate the expression of microRNA542-3p (miR-542-3p) in cancerous tissues of patients with colorectal cancer. Methods Forty colorectal cancer patients were selected for pairing with normal esophageal epithelial tissue, normal human epithelial cell line HIEC and colorectal cancer cell lines Lovo, LS1747, SW620, HT29 and SW480. The expression of miR-542-3p in colorectal cancer tissues, normal intestinal epithelial tissues, intestinal cancer cells and normal intestinal epithelial cell lines was detected by real-time fluorescence polymerase chain reaction. The proliferation of HT29 cells was detected by exogenous transfection of miR-542-3p mimics or inhibitor at 24, 48, 72 and 96 hours after transfection. The migration ability of HT29 cells before and after transfection was evaluated by scratch test to evaluate the effect of miR-542 The biological function of -3p in colorectal cancer cells. Results MiR-542-3p expression was significantly lower in cancerous tissues than in normal intestinal epithelium (P <0.05). MiR-542-3p expression was significantly lower in colorectal cancer cell lines HT29, SW620 and Lovo than in normal intestinal cell lines P <0.05). However, in paired cell lines, the expression was also significantly decreased in highly metastatic colorectal cancer cell lines SW620 and Lovo (P <0.05). There was no significant difference in cell proliferation between miR-542-3p inhibitor group, miR-542-3p mimics group, control group and mimics control group at 24, 48, 72 and 96 h (P> 0.05). After 24 h, the migration ability of miR-542-3p mimics group was significantly lower than that of mimics control group (P <0.05), while the migration ability of miR-542-3p inhibitor group was significantly higher than that of inhibitor control group (P <0.05). Conclusion MiR-542-3p is associated with the invasion and metastasis of colorectal cancer cells and may be used as a molecular marker for invasion and metastasis of colorectal cancer.