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目的:通过观察不对称牵引引起的成年SD大鼠髁突软骨下骨的成骨活性变化,了解关节外不对称机械应力对髁突的影响。方法:选用10周龄雄性SD大鼠20只(随机分为轻力组8只、重力组8只、对照组4只),实验组动物使用关节外固定加力装置,分别施加0.39N和1.18N(轻力组和重力组)的力,加力第28天拆除加力装置,第56天取得标本。在实验前1天、去除牵引装置前1天和处死前1天注射荧光染料,。使用Masson染色和活体荧光技术观察软骨下骨的胶原成熟和钙盐沉积。结果:正常软骨下骨有成骨活动,在负荷加载后,加力侧骨胶原成熟程度高于非加力侧,较小负荷引发的成骨活动更为活跃。结论:成年个体的髁突软骨下骨在外力刺激下出现成骨活性变化,发生适应机械应力环境的重塑,压应力促进胶原成熟,但旋转位移抑制胶原成熟。负荷增加可促进成骨,轻力的促进效应更强。
OBJECTIVE: To observe the changes of osteogenesis activity of subchondral bone of adult SD rats induced by asymmetric traction to understand the effect of asymmetric mechanical stress on condyle. Methods: Twenty male 10-week-old male Sprague-Dawley rats were randomly divided into mild stress group (n = 8), gravitational force group (n = 8) and control group (n = 4) N (light and gravitational group) force, the force on the 28th to remove the force device, the first 56 days to obtain the specimen. Fluorescent dyes were injected one day before the experiment, one day before removal of the distraction device and one day before sacrifice. Masson staining and in vivo fluorescence techniques were used to observe collagen maturation and calcium deposition in the subchondral bone. RESULTS: Normal subchondral bone had osteogenic activity. After loading, the collagen maturation level in the force side was higher than that in the non-force side, and the osteoblast activity induced by the smaller load was more active. CONCLUSIONS: The adult human condyle subchondral bone changes in osteogenic activity under the external force stimulation. The remodeling adapts to the mechanical stress environment and the compressive stress promotes the collagen maturation. However, the rotational displacement inhibits the collagen maturation. Increased load can promote osteogenesis, the promotion effect of light power is stronger.