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Aim: To evaluate the quantitative detection of human telomerase RNA (hTR) and human telomerase reverse tran-scriptase (hTERT) mRNA as diagnostic parameters in the workup of testicular tissue specimens from patients presentingwith non-obstructive azoospermia. Methods: hTR and hTERT mRNA expression were quantified in 38 cryopre-served testicular tissue specimens by fluorescence real-time reverse transcription - polymerase chain reaction (RT-PCR)in a LightCycler(r). This was paralleled by conventional histological workup in all tissue specimens and additionalsemithin sectioning preparation in cases with maturation arrest (n = 12) and Sertoli-cell-only syndrome (n = 12). Re-sults; The average normalized hTERT expression (N_(hTERT)) was 131.9±48.0 copies (mean ± SD) in tissue speci-mens with full spermatogenesis, N_(hTERT) = 51.2 ±17.2 copies in those with maturation arrest and N_(hTERT) = 2.7 ± 2.4copies in those with Sertoli-cell-only syndrome (SCOS). The discriminant analysis showed that detection of
Aim: To evaluate the quantitative detection of human telomerase RNA (hTR) and human telomerase reverse tran-scriptase (hTERT) mRNA as diagnostic parameters in the workup of testicular tissue specimens from patients presenting with non-obstructive azoospermia. Methods: hTR and hTERT mRNA expression were quantified in 38 cryopre-served testicular tissue specimens by fluorescence real-time reverse transcription - polymerase chain reaction (RT-PCR) in a LightCycler (r). This was paralleled by conventional histological workup in all tissue specimens and additional semithinization preparations in cases The average normalized hTERT expression (N_ (hTERT)) was 131.9 ± 48.0 copies (mean ± SD) in tissue speci- fication (n = 12) mens with full spermatogenesis, N hTERT = 51.2 ± 17.2 copies in those with maturation arrest and N hTERT = 2.7 ± 2.4 copies in those with Sertoli-cell-only syndrome (SCOS). The discriminant analysis showed showed that detection of