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目的 了解微量残留白血病 (MRL)在体内的分布状态。方法 利用携带外源性标志基因LacZ的白血病细胞株LT12nl建立了BN大鼠MRL模型。应用逆转录 聚合酶链反应 (RT PCR)及巢式PCR方法 ,结合X gal染色法、细胞形态学及病理形态学方法检测BN大鼠MRL阶段白血病细胞中的特异性标志基因LacZ。结果 化疗后第 4天肱骨标本中出现阳性条带 ;第 6天股骨出现阳性条带 ;第 9天脾脏出现阳性条带 ,肱骨条带的信号明显较前增强。同期X gal检测脾脏阴性。外周血、肝脏持续阴性。结论 PCR方法可作为实验性MRL分布模式研究的有效手段之一。
Objective To understand the distribution of minimal residual leukemia (MRL) in the body. Methods The BN rat MRL model was established using the leukemia cell line LT12nl carrying the exogenous marker gene LacZ. Reverse transcription polymerase chain reaction (RT PCR) and nested PCR were combined with X gal staining, cell morphology and pathomorphology to detect the specific marker gene LacZ in BML rat MRL stage leukemia cells. Results On the 4th day after chemotherapy, there was a positive band in the tibial specimen; on the 6th day, there was a positive band on the femur; on the 9th day, a positive band appeared on the spleen, and the signal on the tibial band was significantly higher than before. During the same period X gal detected spleen negative. Peripheral blood and liver were negative. Conclusion PCR method can be used as one of the effective methods to study the distribution pattern of experimental MRL.