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目的探讨肝细胞癌合并肝硬化患者癌组织、癌旁肝组织ALB mRNA、IGF-1mRNA、IGFBP-3mRNA的表达。方法对24例肝细胞癌合并肝硬化行手术的患者和12例因胆石症或肝血管瘤行手术的患者进行研究。用RT-PCR法检测肝组织(包括癌组织、癌旁组织)ALBmRNA、IGF-1mRNA、IGFBP-3mRNA的表达,肝组织行Ki67免疫组织化染色。结果肝细胞癌合并肝硬化患者癌组织、癌旁肝组织ALB mRNA、IGF-1mRNA、IGFBP-3mRNA表达水平明显低于正常肝组织,且癌组织ALB mRNA、IGFBP-3mR-NA表达水平明显低于癌旁肝组织,而IGF-1mRNA表达水平明显高于癌旁肝组织。肝组织Ki67指数,癌组织、癌旁肝组织明显高于正常肝组织,且癌组织明显高于癌旁肝组织。结论IGF-1和IGFBP-3的不平衡可能与肝硬化患者肝癌的发生、发展有关。
Objective To investigate the expression of ALB mRNA, IGF-1 mRNA and IGFBP-3 mRNA in hepatocellular carcinoma with cirrhosis and adjacent liver tissues. Methods Twenty-four patients with hepatocellular carcinoma complicated by cirrhosis and 12 patients undergoing surgery with cholelithiasis or hepatic hemangiomas were studied. The expression of ALBmRNA, IGF-1mRNA and IGFBP-3mRNA in liver tissues (including cancer tissues and paracancerous tissues) were detected by RT-PCR, and Ki67 immunohistochemical staining of liver tissues was performed. Results The expression of ALB mRNA, IGF-1mRNA and IGFBP-3mRNA in hepatocellular carcinoma with cirrhosis and adjacent liver tissues were significantly lower than those in normal liver tissues, and the expression levels of ALB mRNA and IGFBP-3mR-NA in cancer tissues were significantly lower than those in normal liver tissues Paracancer liver tissue, while the expression of IGF-1mRNA was significantly higher than the adjacent liver tissue. The Ki67 index of liver tissue, cancer tissue and paracancer liver tissue were significantly higher than that of normal liver tissue, and the cancerous tissue was significantly higher than the adjacent liver tissue. Conclusion The imbalance of IGF-1 and IGFBP-3 may be related to the occurrence and development of liver cancer in patients with cirrhosis.