Objective: To investigate whether glucagon like peptide-1 (GLP-1) can reduce the cholesterol in foam cells derived from macrophages by affecting the expression of CD36 and acyl-CoA1 (ACAT1) The content. Methods: Macrophages were obtained from mice, and after corresponding treatment, they were divided into 4 groups: blank control group, experimental group, GLP-1 agitation group, GLP-1 inhibition group, and the expression of CD36, ACAT1 mRNA and protein in cells of each group was detected. Expression and levels of total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE). Results: Compared with the blank control group, the content of TC, FC, CE and the expression of CD36, ACAT1 mRNA and protein in the experimental group were significantly increased (P <0.05); compared with the experimental group, the content and content of TC, FC, CE in the GLP-1 excited group The expressions of CD36 and ACAT1 mRNA and protein were significantly decreased (P <0.05); compared with the GLP-1 agitation group, the contents of TC, FC and CE and the expression of CD36, ACAT1 mRNA and protein were significantly increased in the GLP-1 inhibition group (P <0.05 ). Conclusion: The expression levels of CD36 and ACAT1 can be suppressed by GLP-1, and under the indirect influence of GPL-1, the level of intracellular cholesterol will also be reduced, so that the process of macrophages to foam cells is suppressed Thereby slowing down the development of atherosclerosis.