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目的:研究2011年宁波艾滋病毒(HIV)感染者高效抗逆转录病毒治疗(HAART)效果及耐药变异。方法:收集272例HAART的HIV-1感染者抗凝血,检测CD4+T淋巴细胞和HIV-1病毒载量,病毒载量>1000 copies/ml的样品扩增HIV-1的pol区,测序结果使用斯坦福大学HIV耐药数据库分析。结果:CD4+T淋巴细胞绝对值为311±160个/μl。19份样品病毒载量>1000 copies/ml。10条序列出现耐药突变,总耐药率为3.73%(10/268)。8条产生针对核苷酸类反转录酶抑制剂(NRTI)和非核苷酸类反转录酶抑制剂(NNRTI)的突变。2条只产生了针对NNRTI的突变。结论:宁波HIV抗病毒治疗人群耐药毒株流行处于较低水平,该人群对目前使用的抗病毒药物敏感。但耐药突变呈多样化趋势,应加强耐药监测的研究以减少耐药突变株的产生与传播。
Objective: To study the effect and drug resistance variation of highly active antiretroviral therapy (HAART) in HIV-infected in Ningbo in 2011. METHODS: A total of 272 HAART HIV-1 infected individuals were collected for anticoagulation, CD4 + T lymphocytes and HIV-1 viral load, and samples with viral load> 1000 copies / ml were used to amplify the pol region of HIV-1. Sequencing Results were analyzed using Stanford University HIV drug resistance database. Results: The absolute value of CD4 + T lymphocytes was 311 ± 160 cells / μl. 19 samples had a viral load of> 1000 copies / ml. Ten drug resistance mutations occurred in the 10 sequences, with a total resistance rate of 3.73% (10/268). Eight produce mutations directed against nucleotide reverse transcriptase inhibitors (NRTIs) and non-nucleotide reverse transcriptase inhibitors (NNRTIs). Two of the mutations produced only for NNRTI. Conclusion: The epidemic of drug-resistant strains of HIV-infected patients in Ningbo is at a low level, and the population is sensitive to currently used antiviral drugs. However, drug-resistant mutants showed a trend of diversification. Drug resistance monitoring should be strengthened to reduce the generation and spread of drug-resistant mutants.