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目的探讨柴胡+丹参+五味子(CHAIDANWU)对肝硬化大鼠的作用及其机制。方法复制大鼠CCl4肝硬化模型,给予CHAIDANWU醇提物治疗,同时体外以醇提物和含药血清作用正常与模型大鼠肝细胞。测定血清酶水平、血清与匀浆HYP、PCI、PCIII含量,TNF-α、CD14i、NOS、MMP-1、TIMP-1在肝组织内阳性表达率,TUNEL法检测肝组织细胞凋亡,DNA琼脂糖凝胶电泳检测体外培养肝细胞凋亡,采用标准积分对肝细胞的变性及胶原纤维增生程度进行评价。结果CHAIDANWU能明显地降低血清酶活性,体外试验与体内结果一致。血清和肝组织中PCI、PCIII含量,肝组织TNF-α、CD14i、NOS、MMP-1、TIMP-1阳性率及肝细胞凋亡率明显降低,阻滞肝细胞的变性及胶原纤维增生。结论CHAIDANWU阻滞CCl4诱导大鼠肝硬化的作用,这种作用可能与抑制肝非实质激活,细胞因子释放减少,贮脂细胞释放TIMP-1壁降低,胶原纤维分解加速,肝细胞凋亡数减少有关。
Objective To investigate the effect of Chaihu + Salviae miltiorrhizae + Schisandra on cirrhosis in rats and its mechanism. Methods Rat model of CCl4 liver cirrhosis was reproduced and treated with ethanol extract of CHAIDANWU. Meanwhile, ethanol extract and serum were used to treat normal and model rat hepatocytes. Serum enzyme levels, serum and homogenate HYP, PCI, PCIII levels, positive expression rates of TNF-α, CD14i, NOS, MMP-1 and TIMP-1 in liver tissue were detected. TUNEL method was used to detect the apoptosis of liver tissue. Glucose gel electrophoresis was used to detect hepatocyte apoptosis in vitro. The standard integration was used to evaluate the degeneration of hepatocytes and the degree of collagen fibrosis. Results CHAIDANWU significantly reduced serum enzyme activity, in vitro tests and in vivo results. Serum and liver tissue of PCI, PCIII content, liver tissue TNF-α, CD14i, NOS, MMP-1, TIMP-1 positive rate and hepatocyte apoptosis rate was significantly reduced, blocking the degeneration of hepatocytes and collagen fibrosis. Conclusions CHAIDANWU blocks CCl4-induced hepatic cirrhosis in rats. This effect may be related to the inhibition of hepatic non-parenchymal activation, reduction of cytokine release, reduction of TIMP-1 release by fat-storing cells, acceleration of collagen fiber decomposition and reduction of hepatocyte apoptosis related.