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利用ELISA技术检测自身免疫甲状腺病(AITD)患者周围血单个核细胞(PBMC)体外培养产生甲状腺球蛋白抗体(TGA)。结果表明:体外培养AITD患者PBMC能够产生可测出的TGA,阳性率31.25％。桥本甲状腺炎(HT)较Graves甲亢(GD)更易体外产生TGA,阳性率分别为44.19％和16.22％。美洲商陆刺激体外产生TGA增加,而可溶性甲状腺球蛋白对体外产生TGA无刺激作用。抗甲状腺药物他巴唑能够抑制体外产生TGA。HT患者血清TGA水平与体外产生TGA呈正相关,但未观察到GD患者血清TGA与体外产生TGA的相关性。提示AITD患者体内存在可引起自身抗体产生的免疫调节紊乱、HT与GD在产生自身抗体的机制上可能有所不同。
Thyroglobulin antibody (TGA) was detected by ELISA in peripheral blood mononuclear cells (PBMCs) from patients with autoimmune thyroid disease (AITD). The results showed that TMC could be detected in peripheral blood mononuclear cells of AITD patients with a positive rate of 31.25%. Hashimoto’s thyroiditis (HT) produced more TGA in vitro than Graves’ hyperthyroidism (GD), with positive rates of 44.19% and 16.22%, respectively. Pokeweed stimulates the increase of TGA in vitro, whereas soluble thyroglobulin has no stimulation of TGA in vitro. Anticonvulsant methimazole inhibits TGA production in vitro. The serum level of TGA in HT patients was positively correlated with TGA in vitro, but no correlation was found between serum TGA and TGA in GD patients. It is suggested that there are immunomodulatory disorders that cause autoantibodies in patients with AITD. The mechanism by which HT and GD may produce autoantibodies may be different.