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电化学传感器在用于细胞实时监测过程中,电极界面污染严重影响其检测性能.通过将纳米光催化剂与电化学传感材料复合,构建光致清洁电化学传感器,为电极界面的高效及无损更新提供了新思路.然而光催化产生的活性氧自由基导致细胞损伤,限制了细胞培养及检测过程中电极界面的实时更新.为此,我们在PEDOT@CdS/TiO_2/ITO可见光致更新电极表面旋涂明胶薄层,在保持电极良好的光致清洁和电化学传感性能同时,利用明胶薄层阻碍光催化产生的活性氧自由基扩散至细胞表面,显著降低了细胞损伤.此外,明胶优良的生物相容性有利于细胞的黏附及增殖.利用该电极,我们实现了人脐静脉内皮细胞(HUVECs)培养过程中,电极的在线更新以及细胞释放一氧化氮的实时监测.
Electrochemical sensors are used in real-time monitoring of cells, and the interface contamination of the electrodes seriously affects their detection performance.Compared with electrochemical sensing materials, nano-photocatalysts are used to construct a photo-electrochemical sensor for efficient and non-destructive update of the electrode interface However, photocatalytic generation of reactive oxygen species leads to cell damage, which limits the real-time renewal of the electrode interface during cell culture and detection process.To this end, we investigated the surface rotation of PEDOT @ CdS / TiO_2 / ITO visible light- Gelatin coating thin layer, while maintaining the electrode good photoluminescent and electrochemical sensing properties at the same time, the use of gelatin thin layer blocking the photocatalytic activity of reactive oxygen species diffuse to the cell surface, significantly reducing cell damage.In addition, gelatin excellent Biocompatibility favors cell adhesion and proliferation, and with this electrode we have achieved real-time monitoring of the electrode online update and cell nitric oxide release during culture of human umbilical vein endothelial cells (HUVECs).