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目的:探讨5-氮杂胞苷(5-azacytidine,5-aza)对体外骨髓间充质干细胞(Mesenchymal stem cells,MSCs)细胞增殖与分化潜能的影响。方法:从Wistar大鼠骨髓中获得MSCs,培养传代,透射电镜下观察诱导前后细胞形态变化;MTT法检测5-aza对MSCs生长的影响;免疫细胞化学方法检测Actin(α-Sarcomeric)、Desmin、Myoglobin、NSE、GFAP的表达。结果:5-aza对MSCs有抑制作用,在透射电镜下观察可见有心肌样细胞和神经元样细胞的形态变化。免疫细胞化学检测结果表明,MSCs经5-aza诱导14dactin、desmin、myoglobin、NSE和GFAP的表达均较对照组显著增高,P<0.01。结论:5-aza对MSCs细胞生长有抑制作用,MSCs被5-aza诱导分化成心肌样细胞和神经元样细胞。
Objective: To investigate the effects of 5-azacytidine (5-aza) on the proliferation and differentiation of mesenchymal stem cells (MSCs) in vitro. Methods: MSCs were obtained from the bone marrow of Wistar rats and cultured for passage. The morphological changes of cells were observed under transmission electron microscope before and after induction. The effects of 5-aza on the proliferation of MSCs were detected by MTT assay. Actin (α-Sarcomeric), Desmin, Myoglobin, NSE, GFAP expression. Results: 5-aza could inhibit the proliferation of MSCs. The morphological changes of cardiomyocyte-like cells and neuron-like cells were observed under transmission electron microscope. The results of immunocytochemistry showed that the expression of 14dactin, desmin, myoglobin, NSE and GFAP in MSCs induced by 5-aza was significantly higher than that in control group (P <0.01). CONCLUSION: 5-aza inhibits the growth of MSCs. MSCs are differentiated into cardiomyocyte-like cells and neuron-like cells by 5-aza.