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单个核细胞粘附于动脉内膜表面可见于机体的许多病理过程和正常的生命活动,如炎症、免疫反应、动脉粥样硬化等.但有关粘附的机制尚不清楚,为此,我们对粘附相关蛋白介导粘附的作用进行了系列研究.本实验采用单克隆抗体阻断法,观察了Vn和CD44在内皮一单个核细胞粘附过程中的作用和作用特点.实验采用培养的血管内皮细胞,传至第二至三代,分离纯化外周血单个核细胞,将单个核细胞用单克隆抗体处理后,
The adhesion of mononuclear cells to the intima surface of the artery can be seen in many pathological processes and normal life activities of the body, such as inflammation, immune response, atherosclerosis, etc. However, the mechanism of adhesion is not clear, therefore, we Adhesion-related protein-mediated adhesion in a series of studies.In this experiment, monoclonal antibody blocking method was used to observe the role of Vn and CD44 in endothelial mononuclear cell adhesion process and the role of the characteristics of the experiment using cultured Vascular endothelial cells, passed to the second to third generation, purified peripheral blood mononuclear cells, mononuclear cells treated with monoclonal antibody,