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恶性肿瘤的发生、发展与突变事件密切相关,检出人类在环境中接触的突变剂依赖于致突变试验。以次黄嘌呤鸟嘌呤磷酸核糖基转移酶(hypoxanthine-guanine phosphoribosyl transferase,HPRT)基因为报告基因的HPRT突变试验就是其中几个重要的致突变试验之一,由于其本身所具有的独特生物学特性,逐渐成为基因突变机制和修复机制理想的研究靶点,HPRT基因突变分析法也逐渐成为很有价值并被广泛应用的生物剂量计。但是,HPRT基因突变率的检测也受诸多因素影响,例如高剂量时,射线造成的已不仅仅是单基因损伤,另外,HPRT自发突变频率受年龄、样本数量等的影响。因此,HPRT基因突变频率已不能完全反映损伤程度与剂量之间的关系。
The occurrence and development of malignant tumors are closely related to mutation events. Mutants that detect human exposure in the environment rely on mutagenicity tests. The HPRT mutation test using hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene as a reporter gene is one of several important mutagenicity tests. Due to its unique biological characteristics Has gradually become an ideal research target of gene mutation mechanism and repair mechanism. The HPRT gene mutation analysis method has also gradually become a valuable and widely used biological dosimeter. However, the detection of HPRT gene mutation rate is also affected by many factors, such as high doses, the radiation has caused more than just single-gene injury, in addition, HPRT spontaneous mutation frequency by the age, the number of samples and so on. Therefore, the frequency of HPRT gene mutation can not fully reflect the relationship between the degree of damage and dose.