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在沙壤、中壤和黏壤3种质地土壤条件下,以优质强筋小麦品种山农12为材料研究了小麦强势与弱势籽粒高分子量谷蛋白亚基(HMW-GS)积累特征及其与谷蛋白大聚合体(GMP)粒度分布的关系。结果表明,3种质地土壤上,小麦强、弱势籽粒HMW-GS花后14d均已形成,强势粒HMW-GS含量明显高于弱势粒,说明强势粒具有较强的HMW-GS积累能力。小麦籽粒HMW-GS含量和GMP含量均表现为黏壤土>中壤土>沙壤土,说明黏壤土有利于HMW-GS的积累。小麦GMP粒径分布范围在0.37~245μm之间;数目分布呈单峰曲线,体积和表面积分布呈双峰曲线。小麦强势粒GMP>100μm颗粒数目百分比和体积百分比均显著高于弱势粒,强势粒具有更多的大粒径GMP颗粒。小麦HMW-GS含量和GMP含量与<10μm和<100μmGMP颗粒体积百分比均呈显著或极显著负相关,与>100μmGMP颗粒体积百分比呈显著或极显著正相关,说明大粒径GMP颗粒具有较高的HMW-GS含量。
Under the condition of sandy soils, mid-soils and clay soils, the high-gluten wheat cultivar Shannong 12 was used to study the accumulation characteristics of high molecular weight glutenin subunits (HMW-GS) Relationship between particle size distribution of large gluten polymer (GMP). The results showed that HMW-GS of strong and weak grains of wheat was formed on the 14th day after flowering, HMW-GS of strong grains was obviously higher than that of weak grains, which indicated that strong grains had strong accumulation ability of HMW-GS. HMW-GS content and GMP content of wheat grain all showed as clay loam soil> medium loam soil> sandy loam soil, which indicated that clay loam was beneficial to HMW-GS accumulation. Wheat GMP particle size distribution range between 0.37 ~ 245μm; the number distribution showed a single peak curve, volume and surface area distribution showed a bimodal curve. The grain number percentage and the volume percentage of GMP> 100μm were higher than those of inferior grains, while that of strong grains had more GMP particles. The HMW-GS content and GMP content in wheat were significantly or very significantly negatively correlated with the volume fraction of GMP particles <10μm and <100μm, and significant or very significant positive correlation with the percentage of> 100μm GMP particles, indicating that the GMP particles with large particle size had higher HMW-GS content.